Cytokinin dehydrogenase precursor
WebMay 15, 2012 · Cytokinins (CKs) are a class of plant hormones that play a central role during plant cell growth and differentiation, thus, influencing numerous developmental programs including root growth and branching, control of apical dominance in the shoot, anthesis, seed development, leaf senescence and chloroplast development ( Kakimoto, 2003, … Webendogenous cytokinin concentrations are nanomolar [15]. Enzyme based amperometric biosensors provide a simple, fast, selective, convenient and low cost analytical technique. Cytokinin dehydrogenase (CKX; EC 1.5.99.12) degrades cytokinins by oxidative cleavage of N6- side chain using FAD as the electron-donating cofactor [16–19].
Cytokinin dehydrogenase precursor
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To identify an omnipresent FAD-dependent protein, that could be related to the cytokinin binding domain we performed a pBlast search. By application of pBlast search, we have found that on the sequence level the closest to CKX protein in Arabidopsis thalianais the FAD-linked D-2-hydroxyglutarate … See more To track the evolutionary origin of the CKXs, we have searched for the homologous sequences from the Uniprot, Pfam, and NCBI databases using the homology-based … See more Because our phylogenetic tree analysis could not answer the question regarding the origin of the cytokinin binding domain, we have analysed structural models of the CK-binding domains. … See more Domain architecture plays a key role in understanding the functionality of proteins along with their evolutionary history. To evaluate the gain … See more To understand the evolutionary history of the C-terminal cytokinin binding domain proteins the phylogenetic trees were inferred with the … See more WebApr 11, 2024 · In addition to cytokinin, Zn deficiency also represses the levels of auxin and gibberellic acid (GA) in wheat and maize (Sekimoto et al., 1997) and abscisic acid (ABA) levels in rape leaves (Cakmak et al., 1989), whereas it induces the accumulation of 1-aminocyclopropane-1-carboxylic acid (ACC), a precursor of ethylene biosynthesis in …
WebJan 19, 2024 · OsGRF4 regulated two cytokinin dehydrogenase precursor genes (CKX5 and CKX1) resulting in increased cytokinin levels, which might affect the panicle traits (Sun et al. 2016). SP1 contains a conserved PTR2 domain composed of 12 transmembrane domains, which can regulate the activity of the spike meristem, leading to the short … WebSince 1980s, it has already been established that the enzyme, cytokinin oxidase/dehydrogenase (CKO/CKX) deactivates cytokinins; however, the potential …
WebJan 31, 2024 · Cytokinin is first perceived by hybrid histidine kinase (HK) receptors, mainly localized in endoplasmic reticulum (ER), and results in autophosphorylation … WebSep 1, 2024 · In plants, cytokinin ribonucleosides mainly exist in forms of iPR and tZ ribonucleoside (tZR). They are considered as precursors and mainly converted to their active form via LOG after phosphorylation. The logseptuple mutants exhibited severe cytokinin-deficient phenotypes [14].
WebDec 7, 2015 · Here, we show that the Lotus japonicus Ckx3 cytokinin oxidase/dehydrogenase gene is induced by Nod factor during the early phase of nodule initiation. ... Quantification was obtained using a multiple reaction-monitoring mode of selected precursor ions and the appropriate product ion. GenBank accession numbers: …
WebJan 1, 2001 · Abstract. An enzyme degrading cytokinins with isoprenoid side chain, previously named cytokinin oxidase, was purified to near homogeneity from wheat and barley grains. New techniques were developed for the enzyme activity assay and staining on native electrophoretic gels to identify the protein. The purified wheat enzyme is a … camp chef explorer partsWebApr 13, 2024 · In vitro experiments to induce Rafflesia seed germination using various plant growth regulators such as gibberellins, ethylene, brassinolide, and cytokinin have also been unsuccessful (Molina et al., 2024). Even the synthetic strigol, GR24, known to stimulate seed germination in plant parasites of Orobanchaceae, did not induce germination. camp chef everest griddleWebAug 27, 2024 · Cytokinins are mobile multifunctional plant hormones with roles in development and stress resilience. Although their Histidine Kinase receptors are … first strawberry shortcake dollWebJun 1, 2024 · Cytokinins maintain the growth potential (pluripotency) of shoot apical meristems, which provide stem cells for the generation of leaf primordia during the … camp chef everest high-output 2-burner stoveWebOct 28, 2024 · Cytokinin is a phytohormone involved in the regulation of diverse developmental and physiological processes in plants. Its potential in biotechnology and for development of higher-yield and more resilient plants has been recognized, yet the molecular mechanisms behind its action are far from understood. In this report, the roots … camp chef dlx start upWebOct 4, 2024 · As cytokinin is a key regulator for the maintenance of shoot apical meristem, deepening our understanding of the regulatory mechanisms of cytokinin biosynthesis and transport in response to … first strawberryWebMay 1, 2005 · Cytokinin dehydrogenase was confirmed to be present in the apoplast of cells. Co-staining of enzyme activity for laccase, an enzyme poised to function on the cell wall in the apoplast, in those tissues suggests a possible cooperation of the enzymes in cytokinin degradation. camp chef everest 2 burner propane stove