WebFigure 3 .Total RNA was purified from 10 1-10 3 HeLa cells using the Total RNA Mini Kit Blood/Cultured Cell (2 replications of each cell number). The purified RNA was eluted with 25 μl RNase-free Water. cDNA synthesis was carried out with a 10 μl aliquot of purified RNA (2/5) using a Transcriptor First Strand cDNA Synthesis Kit (Roche) in a final volume of 20 … Web1 jan. 2024 · The absolute amount of miR-21 in 20 ng of HeLa total RNA sample was 64.6 amol, which was well consistent with result of our Cas13a-TFCN method (absolute amount of miR-21 was 69.1 amol). These results indicated that the proposed assay worked well for miRNA quantification in the real biological sample, and it can be used as a promising tool …
Requirement of transcription-coupled nucleotide excision repair …
Web8 apr. 2024 · RNA from human tissues was commercially obtained. First Choice Total RNA from human brain (Cat No. AM7962; Lot No. 1799894), skeletal muscle (Cat No. … Web16 sep. 2004 · Total RNA from HeLa cells was used for selective enrichment using the cap-binding protein eIF4E. RNA was extracted from the bound (B) or unbound (UB) fraction. Because the whole RNA from each fraction was used for the assay without further normalization, it corresponds to 100% of the input. bsc biotechnology colleges in odisha
Hela Total Rna Thermo Fisher Bioz
Web12 apr. 2024 · (B) Summary data for ASO screen by free uptake in patient-derived (SU-DIPG-XIII) neurosphere cultures using RT-qPCR of total RNA extracted after 5 days (n = 3). ( C ) Dose-response experiment with cotransfected minigenes in HeLa cells, with representative RT-PCR gel on the left and quantification on the right ( N = 3). Web14 apr. 2024 · According to the manufacturer’s instructions, the total RNA was isolated from each TIC tissue using TRIZOL ... HeLa cells were seeded into 24-cell plates and transfected 24 h later. The miRNA-326 was co-transfected with 100 ng of psicheck2-NKX3.2-fragment and 40 pmol of miRNA-326 mimics or negative control ... WebThe Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. excel shrink row height to fit