Irf8 flox mouse

Author: yddl

August undefined, 2024

WebDuring our analyses, we also examined littermate mice with a genotype of floxed IRF8 only, PU.1 only, or IRF8/PU.1 heterozygous with or without Mb1-Cre (SI Appendix, Table S1). While Mb1-Cre–mediated PU.1 deletion alone did not affect B cell distribution in the BM, Mb1-Cre–mediated IRF8 deletion alone appeared to induce a moderate reduction ... WebSecukinumab (anti-IL-17A) (Cosentyx, AIN457) is a human IgG1κ monoclonal antibody that binds to the protein interleukin (IL)-17A, and is applied for the treatment of psoriasis, ankylosing spondylitis, and psoriatic arthritis. MW=147.9 kDa. Selleck's Secukinumab (anti-IL-17A) has been cited by 2 Publications

IRF8 is involved in cutaneous wound healing in mice and patients.

WebMyeloid cell-specific IRF8 conditional knockout mice (IRF8flox/flox; Lyz2Cre/+) with a C57BL/6 background were generated by mating IRF8-flox mice (Feng et al. 2011) with Lyz2-Cre knock-in mice. The primers used for genotyping are shown in Supplementary Table S3. The genotyping results are shown in Supplementary Figure S4a. Harvesting of blood ... WebIRF8 (interferon regulatory factor 8) is a transcriptional activator or repressor. It specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes. … devil\u0027s pizza durham nc 9th street

Onecut-dependent Nkx6.2 transcription factor expression is …

WebMar 8, 2024 · SSc remain unknown. IRF8 is a transcriptional regulator that is essential for the differentiation and function of monocytes and macrophages and thus may be involved in the regulation of macrophage phenotypes in SSc fibrosis. In this study, we measured IRF8 levels in circulating monocytes of 26 patients with SSc (diffuse cutaneous SSc, WebProduct Specific Information. Description: The V3GYWCH monoclonal antibody reacts with human and mouse interferon regulatory factor 8 (IRF8; ICSBP). IRF8 is a 50 kDa transcription factor that plays a critical role in the development of dendritic cells (DC). Specifically, IRF8 is essential in the development and differentiation of plasmacytoid ... WebInterferon regulatory factor 8 (Irf8), also known as interferon consensus sequence-binding protein, is a component of the Irf transcription factor family. Irf8 is differentially expressed … churchill audio

The macrophage IRF8/IRF1 regulome is required for protection …

WebDec 11, 2024 · Mice with IRF8 deficiency only in colon epithelial cells (termed IRF8cKO) were created by crossing Irf8-floxed mice with villin-cre mice (Figures S1A and S1B) with the deletion of exon 2 (Figure S1C). As reported in the literature, we observed that IRF8KO mice exhibit elevated levels of CD11b + Gr1 + MDSCs as compared to wild-type (WT) mice. … WebFeb 11, 2024 · Mechanistically, loss of SENP3 increases interferon regulatory factor 8 (IRF8) SUMO3 modification at the K310 amino acid site, which upregulates expression of the nuclear factor of activated T cell c1 (NFATc1) and osteoclastogenesis. churchill at warWebInterferon regulatory factor (IRF) 8 is a transcriptional regulator that is essential for the differentiation and function of monocytes and macrophages, and thus may be involved in the regulation... churchill aurora borealis live cam

"WebJun 21, 2024 · IRI was induced in Irf8-deficient mice (Irf8 fl/fl Clec9a cre mice) and control mice (Irf8 fl/fl Clec9a wt mice). (A) mRNA expression of chemokine receptors C-C … " - Irf8 flox mouse

Irf8 flox mouse

The macrophage IRF8/IRF1 regulome is required for protection …

WebFeb 1, 2011 · IRF8-null mice (IRF8 −/ ... Fig. 3A illustrates the strategy for conditional deletion of exon 2 of Irf8. The floxed and WT alleles were detected by PCR in genomic DNA . After crossing with CD19-Cre mice, the homozygous Irf8 f/f mice bearing the Cre gene exhibited a complete depletion of IRF8 protein in splenic B cells . In the BM ... WebIrf8 cKO mice were generated by crossing Irf8 fl/fl mice with Csf1r cre mice, a monocyte/macrophage targeting Cre driver mice. ... Monocyte Subsets with High Osteoclastogenic Potential...

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WebCopies of IRF8 flox or delta locus/ reference gene locus CD45.2 (normalized) ... Mononuclear phagocyte composition in Itgax-cre.Irf8fl/fl or fl/- and Irf8fl/fl mice and effect of Irf8 haplo-insufficiency on intestinal DC subset composition, related to Figure 1. (A and C) Representative flow cytometry plots and (B and D) ... WebIrf8 −/ mice exhibited multiple deficiencies in myeloid and lym-phoid systems, including excessive generation of myeloid cells and diminished Th1 immune responses (37–39) …

WebFeb 14, 2014 · Here we show that IRF8 is downregulated in both mouse hearts and cardiomyocytes after hypertrophic stress treatment. Moreover, IRF8-deficient mice reveal an aggravated pressure... WebUpon arrival, some Irf8 f mice were bred with B6.Cg-Tg(Sox2-cre)1Amc/J mice (Stock No. 008454) for germline removal of floxed exon 2. Offspring with pan deletion of floxed exon …

WebJan 1, 2014 · Here we show that IRF8 is downregulated in both mouse hearts and cardiomyocytes after hypertrophic stress treatment. Moreover, IRF8-deficient mice reveal an aggravated pressure overload-induced hypertrophic response, whereas the opposite effects occur in IRF8-overexpressing mice. WebMice in which the gene expressing B 2 receptor has been specifically deleted from the endothelium (B 2 flox/flox.Tie2 Cre) presented normal blood pressure readings compared …

WebThe Irf8 floxed targeted mutated mouse strain can be used to selectively ablate expression of IRF8 in any cell type in which a Cre recombinase gene is activated. This will permit the...

WebIRF8 is a transcriptional regulator that is essential for the differentiation and function of monocytes and macrophages and thus may be involved in the regulation of macrophage … churchill auctions wallingfordWebIRF8 and IRF1 are transcriptional regulators that play critical roles in the development and function of myeloid cells, including activation of macrophages by proinflammatory signals … devil\u0027s pool at victoria fallsWebMar 8, 2024 · Because IRF8 may contribute to SSc progression, including fibrosis, vascular abnormalities, and impaired wound healing, we evaluated IRF8 levels in SSc monocytes … churchill auroraWebOct 1, 2024 · The IRF8 FLOX mice (C57BL/6J background) were kindly provided by professor Hongliang Li (Wuhan University, China). The hepatocyte-specific IRF8-knockout mice … churchill aurora tourhttp://www.dukecancerinstitute.org/transgenic-and-knockout-mouse churchill audiobookWebFigure Legend Snippet: Upregulation of downstream targets of ERK pathway. A. Western Blot analysis of protein extract from normal appearing mucosa obtained from small intestines tissues from LSL-K-RasG12D and Villin-Cre/LSL-K-RasG12D mice. 10 ug of total protein extracts from LSL-K-RasG12D and Villin-Cre/LSL-K-RasG12D were analyzed by Western … devil\\u0027s powerWeb014175 B6.Cg- Irf8 tm1.1Hm /J This Irf8 f floxed targeted mutation strain may be useful in generating conditional mutants for studying B cell differentiation, immunodeficiency … devil\u0027s pool deaths