Lamp2b-ha
Tīmeklis2024. gada 10. nov. · To produce sEVs RVG -siRNA, a recombinant vector, pcDNA-RVG10-Lamp2b-HA, was constructed and transfected into human embryonic kidney (HEK) 293T cells. The secreted sEVs were isolated from the culture supernatants by differential ultracentrifugation, as previously described. 16 Tīmeklis2024. gada 10. febr. · Plasmid DNA (pDNA), which expressed RBP-Lamp2b-HA, was transfected to 293 T cells and a stable cell line with pDNA was established by selection with hygromycin. Exosomes from the cells were purified and the presence of RBP-Lamp2b-HA on the exosomes was confirmed by a western blot assay with anti-HA …
Lamp2b-ha
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Tīmeklis2015. gada 5. febr. · The Lamp2b fusion proteins were expressed at similar levels in cells, as indicated by the HA Western blot. However, the FLAG peptide on FLAG-Lamp2b-HA could not be detected, although the C-terminal FLAG peptide of Lamp2b-HA-FLAG was detected ( Fig. 1E ). The same pattern was observed in exosomes ( … TīmeklisSequences pcDNA GNSTM-3-RVG-10-Lamp2b-HA Sequences (3) Addgene Sequences: Partial (2) Depositing Scientist Sequences: Full (1) Full Sequences from …
TīmeklispMEH60 Lamp2b MS2 HA (Plasmid #168209) Print Enlarge View all sequences Purpose Mammalian expression of lysosome-associated membrane protein 2b fused … Tīmeklis2024. gada 24. dec. · Mutations in lysosomal-associated membrane protein 2 ( LAMP-2) gene are associated with Danon disease, which often leads to cardiomyopathy/heart failure through poorly defined mechanisms. Here, we identify the LAMP-2 isoform B (LAMP-2B) as required for autophagosome–lysosome fusion in human …
TīmeklisTo test this hypothesis, HEK293FT cells were transfected with Lamp2b containing a C-terminal HA tag (Lamp2b-HA), two C-terminal tags (Lamp2b-HA-FLAG), or an N … TīmeklisWestern blot analysis shows expression of fusion protein CAP-Lamp2b-HA in total protein isolated 36 hours after electroporation of CAP-Lamp2b with BM-MSCs as both the Hemagglutinin HA-tag antibody specific and Lamp2b-specific positive signal shows in the 1 st western blot run ( .
TīmeklisLamp2b-HA and T7-Lamp2b-HA contained the HA tag as fusion proteins of Lamp2b. GAPDH was detected as an internal control. CD63 was detected as an exosome …
Tīmeklis2024. gada 23. maijs · To produce CTL-Exo and CTP-Exo, HEK 293 cells were transfected with FLAG-Lamp2b-HA or CTP-FLAG-Lamp2b-HA plasmids, respectively. To monitor plasmid expression and exosome uptake, mCherry-CD81 plasmids were co-transfected with Lamp2b plasmids (Fig. 1 B). CTL-Exo and CTP-Exo were purified … atandwa kani divorceTīmeklis860 Centennial Ave. • Piscataway, NJ 08854 USA • 1-732-885-9188, 1-877-436-7274 (Toll Free) • [email protected] asif kamal md paTīmeklispcDNA GNSTM-3-Flag-10-Lamp2b-HA (Plasmid #71293) Print Enlarge View all sequences Purpose Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag. Depositing Lab Joshua Leonard Publication asif kamalTīmeklispcDNA GNSTM-3-RVG-10-Lamp2b-HA : 出品公司: 爱迪基因: 目录编号: 71294: 存储实验室: Joshua Leonard: 载体骨架基本信息: 载体名称: pcDNA 3.1+ Hygro: 载体出品 … atanessianTīmeklis2015. gada 3. marts · Exosomes and cells were incubated for 2 h at 37 °C, as described (11). Cells were then washed with PBS and harvested for flow cytometry. Flow … atanfLysosome-associated membrane protein 2 (LAMP2), also known as CD107b (Cluster of Differentiation 107b) and Mac-3, is a human gene. Its protein, LAMP2, is one of the lysosome-associated membrane glycoproteins. The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. … asif kamranTīmeklispcDNA GNSTM-3-RVG-10-Lamp2b-HA (Plasmid #71294) Print Enlarge View all sequences Purpose Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, … atanex