Mcherry-gfp-lc3b

Author: eynd

August undefined, 2024

WebLenti-mCherry-EGFP-LC3B (C3002)和Lenti-EGFP-LC3B (C3003)适合用于一些腺病毒较难感染或者需要筛选稳定细胞株的情况；Ad-mCherry-GFP-LC3B (C3011)和AdPlus-mCherry-GFP-LC3B (C3012)由于表达起始时间短，适合用于能被腺病毒感染并且希望快速获得实验结果的情况，也可以在表达E1的HEK293A、HEK293等适当细胞中扩增，便于后续 ...

R26-CAG-LSL-mCherry-EGFP-LC3

WebpCMV-mCherry-p62是碧云天研发的在哺乳动物细胞中表达红色mCherry标签的人源p62融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表 … Web22 jul. 2024 · The eGFP-LC3B MFI of saponin-treated eGFP-LC3B expressing HeLa (A), HEK293T (B), Jurkat (C), or THP-1 (D) cells was quantified using flow cytometry and the … movies in theaters upcoming

Autophagy restricts Mycobacterium tuberculosis during acute …

Webof the GFP fluorescent signal to acidic conditions of the lysosome lumen, leading to its inactivation compared with the mCherry fluorescence, which is more stable. Thus, colocalization of GFP and mCherry fluorescence (yellow puncta) indicates that the tandem protein is not localized in compartments fused with a lysosome (i.e., on the phagophore or Web29 rijen · Ad-mCherry-GFP-LC3B是碧云天自行研发的重组腺病毒，感染后能够在靶细胞中有效表达红色荧光蛋白mCherry、绿色荧光蛋白 (GFP)和LC3B的融合蛋白，呈现明亮的 … Web3 apr. 2024 · Virus particles were collected after 48 h infection, filtered through a 0.22 μm membrane, added to HCT116 cells, and then incubated for 24 h at 37 °C. The medium was replaced and HCT116 cells were selected for puromycin tolerance (5 μg/mL) to obtain Caspase 3 knock-down, GFP-LC3B, or mcherry-EGFP-LC3B overexpression cells. heather westphal az

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Autophagic Flux Detection: Significance and Methods Involved

Web2 jun. 2011 · A tandem fusion of mCherry and GFP is fused to LC3B (one of the several members of the mammalian LC3 family) to make a pH-sensitive sensor that is used to … Web14 apr. 2024 · Stable MCF10A cell lines carrying pBABE-puro-mCherry-EGFP-LC3B were established as described previously in the section of generation of stable cell lines. Cells were imaged using a Zeiss LSM 780 microscope with a 63X oil immersion objective lens, as described previously in the confocal section. mCherry and EGFP signals were analyzed … movies in theaters yakima waWeb13 apr. 2024 · For Airyscan confocal live-cell imaging, MatTek dishes containing infected cells producing GFP–SEPT6 and mCherry–LC3B were placed on a temperature-regulated chamber (37°C and 5% CO 2) and imaged using a 63×/1.4 C-Plan Apo oil immersion lens on a Zeiss LSM 880 confocal microscope driven by ZEN Black software. movies in theatre in december

"Web10 apr. 2024 · Main. Macro-autophagy (hereafter termed ‘autophagy’) encapsulates large intracellular cargos in a double-membrane structure named the autophagosome 1, 2. Upon fusion of the autophagosome with ... " - Mcherry-gfp-lc3b

Mcherry-gfp-lc3b

Palmitate impairs the autophagic flux to induce p62-dependent

WebHepG2 cells were transduced with mCherry-GFP-LC3 lentiviral vectors, which were pre-packed with the lentivirus packaging kit. The quenching properties of the GFP fluorescent signal in acid conditions (pH < 5) and the cherry fluorescent signal do not differ significantly in different acidic environments [ 28 ] when used to detect autophagic flux. WebTandem GFP-mCherry-LC3B expression indicates elevated autophagic flux in EGFRvIII + (K) BS153 and (L) DKMG cells during hypoxia (24 h O 2 0.02%). Scale bars: 2 μm. …

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WebTo monitor the autophagic sequestration of p62- and LC3-positive bodies, we developed a novel pH-sensitive fluorescent tag consisting of a tandem fusion of the red, acid-insensitive mCherry and the acid-sensitive green fluorescent proteins. This approach revealed that p62- and LC3-positive bodies are degraded in autolysosomes. WebAd-mCherry-GFP-LC3B 关键词：Ad-mCherry-GFP-LC3B,YT160,百奥莱博 ·Tubulin抗体编号：YT156 英文名称：Tubulin antibody 规格：>40次本Tubulin抗体为进口分装，用纯化的微管蛋白作为抗原制备而成的抗Tubulin小鼠单克隆抗体。克隆号为B-5-1-2。本Tubulin抗体识别α-tubulin的C-terminal。

WebpCMV-mCherry-GFP-LC3B是碧云天研发的在哺乳动物细胞中表达红色mCherry和绿色GFP双标签的人源LC3B融合蛋白的质粒。该质粒含有CMV启动子，为卡那霉素抗性，转染后能够在靶细胞中高效表达带有红色荧光蛋白mCherry和绿色荧光蛋白GFP双重标签的LC3B的融合蛋白，呈现明亮的 Web14 okt. 2024 · Ad-mCherry-GFP-LC3B was used to demonstrate blockage of the autophagic flux in eukaryocytes . Infection of Ad-mCherry-GFP-LC3B resulted in overexpression of tandem mRFP/mCherry-GFP proteins. In cells lacking an autophagic state, mCherry-GFP-LC3B was present in the cytoplasm as diffuse yellow fluorescence …

http://www.biofeng.com/adjy/zhili/pBABE-puro%20mCherry-EGFP-LC3B.html WebmCherry-GFP-LC3B慢病毒 mCherry-GFP-LC3B腺相关病毒四．自噬双标系统的工作原理未发生自噬的细胞及含有自噬体的细胞中，由于mCherry与GFP共同表达，细胞呈现黄色荧光。当自噬体与溶酶体融合形成自噬溶酶体后，酸性的溶酶体环境使酸敏感的GFP荧光淬灭，而mCherry不 ...

Web(F and G) GFP-LC3B-expressing HeLa cells were infected with mCherry-expressing Salmonellae. Correlative cryo-ET captures early phagophores (F) and expanded phagophores (G) around a damaged SCV.

WebAutophagy Sensor RFP-GFP-LC3B, and chloroquine diphosphate: Product Type: Autophagy Sensor: Compatible Cells: Mammalian Cells: Excitation/Emission: TagRFP: … heather w figure skating scheduleWebGreen fluorescent protein (GFP)-LC3B adenovirus, GFP-red fluorescent protein (RFP)-LC3B adenovirus and mCherry-EGFP-LC3 adeno-associated virus were obtained from Hanbio (Shanghai, China). Cell Culture. The human HSC cell line LX-2 (Procell, Wuhan, China) was cultured in DMEM supplemented with 10% FBS at 37 °C and 5% CO 2. heather westphal state farm agentWebFUW mCherry-GFP-LC3 (Plasmid #110060) Print Enlarge View all sequences Purpose To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes … movies in theaters wenatchee waWeb7 nov. 2015 · Plasmids construction pQCXIP-tfLC3B expressing mCherry-EGFP tandem ﬂuorescent- tagged LC3B follows.First, mCherry gene ampliﬁedfrom pmCherry-C1 (Clontech, CA, USA) tem-plate PCR(up primer: -GTCGCGGCCGCCACCATGGTGAGCAA-GGGCGAGGAG-3 downprimer: -GCTGGATCCACCTCCGCTTCCA … heather wezenskyWeb11 mei 2024 · mCherry (红光)-eGFP (绿光)-LC3B 串联荧光蛋白可用于检测自噬流水平的融合蛋白。它利用了酸性自溶酶体和中性自噬体之间的 pH 差异以及不同荧光素对 pH 的敏感性差异，以监控从自噬体到自溶酶体的进程 (图 6a)。图 6. 串联荧光探针及荧光共定位检测自噬流【a: mCherry-eGFP-LC3B 串联荧光探针示意图与机制 [6]；b: p27+/+ 和p27-/- … heather whalen raleigh ncWebDescription. pCMV-mCherry-GFP-LC3B is a plasmid developed by Biyuntian to express the red mCherry and green GFP double-labeled human LC3B fusion protein in mammalian cells. This plasmid contains the CMV promoter and is kanamycin resistant. After transfection, it can efficiently express the fusion protein of LC3B with the double label of … heather w goffWeb29 sep. 2024 · mCherry (红光) -eGFP (绿光) -LC3B 串联荧光蛋白可用于检测自噬流水平的融合蛋白。它利用了酸性自溶酶体和中性自噬体之间的 pH 差异以及不同荧光素对 pH 的敏感性差异，以监控从自噬体到自溶酶体的进程 (图 6a) 。图 6. 串联荧光探针及荧光共定位检测自噬流【a: mCherry-eGFP-LC3B 串联荧光探针示意图与机制[6]；b: p27+/+ 和p27-/- … heather wettlaufer wyoming model